Mr Ten Divergence Betwixt Cosmid In Addition To Lambda Vectors
The deoxyribonucleic acid that carries the desired genes to the host jail cellular telephone is called gene cloning vector . It is also known every bit cloning vector, vector, cloning vehicle or carrier DNA. Plasmids, viral DNAs as well as cosmids are used every bit cistron cloning vectors. Cosmid is a derived vector . Cosmid was rootage constructed yesteryear Collins as well as Hohn inwards 1978. Cosmid is an artificial plasmid containing 'cos' sites of lambda (λ) DNA. It is formed yesteryear joining ends of a linearlized plasmid deoxyribonucleic acid amongst ‘cos’ sites of a lambda (λ) DNA. It has an origin of replication, selectable markers as well as cistron cloning sites of the plasmid DNA. They lack structural as well as regulatory genes of λ DNA. Hence, at that spot is no lysis as well as integration of cosmid deoxyribonucleic acid inwards the host cell. Example: Col EI cosmid, pHC 79, pJB8, pWE cosmid etc. Lambda Phage DNA tin compo...