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Showing posts with the label Southern Blotting vs Northern Blotting

Mr X Ten Differences Betwixt Southern Blotting Too Western Blotting

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Southern blotting:  The Southern blot is used to let on the presence of a item deoxyribonucleic acid fragment inward a sample. The technique was developed past times E.M. Southern inward 1975. Western Blotting:  Technique for detecting specific proteins separated past times electrophoresis past times role of labelled antibodies.   The technique was developed past times Towbin et al inward 1979.   Southern Blotting VS  Western Blotting Southern Blotting Western Blotting Technique for the identification of specific deoxyribonucleic acid inward a sample Technique for the identification of specific poly peptide inward a sample Developed past times E.M. Southern hence called “ Southern” The term “Western” has no scientific significance but a misnomer to check amongst Southern blotting Principle is Hybridization; the physical care for of forming a double-stranded deoxyribonucleic...

Mr 10 Deviation Betwixt Southern In Addition To Northern Blotting

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Sothern blotting The technique was developed past times E.M. Southern inward 1975. The Southern blot is used to discovery the presence of a item deoxyribonucleic acid fragment inward a sample. The deoxyribonucleic acid detected tin live on a unmarried gene, or it tin live on purpose of a larger slice of deoxyribonucleic acid such every minute a viral genome. Northern blotting The technique was developed past times Alwine in addition to his colleagues inward 1979 Technique for detecting specific RNAs separated past times electrophoresis past times hybridization to a labeled deoxyribonucleic acid probe.   Southern blotting Northern blotting Southern cite of inventor Northern a misnomer Separation of deoxyribonucleic acid Separation of RNA Denaturation needed Denaturation  non needed Nitrocellulose filter membrane Amino benzyloxymethyl filter newspaper Membrane DNA-DNA  Hybridization RNA-DNA Hybridization