Mr Ten Divergence Betwixt Cosmid In Addition To Lambda Vectors

The deoxyribonucleic acid that carries the desired genes to the host jail cellular telephone is called gene cloning vector. It is also known every bit cloning vector, vector, cloning vehicle or carrier DNA. Plasmids, viral DNAs as well as cosmids are used every bit cistron cloning vectors.

  Cosmid is a derived vector. Cosmid was rootage constructed yesteryear Collins as well as Hohn inwards 1978. Cosmid is an artificial plasmid containing 'cos'  sites of lambda (λ) DNA. It is formed yesteryear joining ends of a linearlized plasmid deoxyribonucleic acid amongst ‘cos’ sites of a lambda  (λ) DNA.  It has an origin of replication, selectable markers as well as cistron cloning sites of the plasmid DNA. They lack structural as well as regulatory genes of λ DNA. Hence, at that spot is no lysis as well as integration  of cosmid deoxyribonucleic acid inwards the host cell.

The deoxyribonucleic acid that carries the desired genes to the host jail cellular telephone is called MR X Difference betwixt Cosmid as well as Lambda vectors

Example: Col EI cosmid, pHC 79, pJB8, pWE cosmid etc.

Lambda Phage DNA tin comport deoxyribonucleic acid fragments as well as integrate into chromosomal deoxyribonucleic acid of host cell. Therefore, it is used every bit a vector for cistron cloning. The constructed lambda deoxyribonucleic acid vectors are of 2 types:
  • Insertation vectors: Target deoxyribonucleic acid is inserted into the vector.
  • Replacement vectors: Target deoxyribonucleic acid replaces a slice of lambda cloning vector
Lambda Phage deoxyribonucleic acid vs Cosmid
Cosmid Vectors
Lambda vectors
Insert size is 45-50 kb Insert size is 20-25 kb
 
Less clones required for consummate library.
More clones required for consummate library. 

Cosmid  libraries are hard to make
Libraries are easier to make.

Cosmid libraries are to a greater extent than hard for screening as well as less efficient.
Libraries are simpler for screening as well as to a greater extent than efficient too.

Colony hybridisation technique is used.
Plaque hybridisation technique is used.

Yield of recombinants per micro grams of starting starting deoxyribonucleic acid is lower amongst cosmid vector.
Yield of recombinants per mg of starting deoxyribonucleic acid is higher amongst lambda vectors.

With cosmid vectors less steps are required to walk along the genome.
With lamda vectors to a greater extent than steps are required to walk along the genome.

Long eukaryotic genes tin last included inwards a unmarried deoxyribonucleic acid fragment that is cloned.
Long eukaryotic cistron may non last included inwards a unmarried deoxyribonucleic acid fragment that is cloned.

Cosmid libraries are maintained inwards aeroplane of  transformed bacteria. If each bacterium population carries the recombinant cosmid, 3,50,000 transformants are to last generated.
Lambda stock consisting library  of recombinant  clones inwards full of DNA. It is concentrated yesteryear CsCl density gradient. Maintenance of phage socks is tedious.

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