Mr 10 Deviation Betwixt Southern In Addition To Northern Blotting
Sothern blotting
Northern blotting
- The technique was developed past times E.M. Southern inward 1975.
- The Southern blot is used to discovery the presence of a item deoxyribonucleic acid fragment inward a sample.
- The deoxyribonucleic acid detected tin live on a unmarried gene, or it tin live on purpose of a larger slice of deoxyribonucleic acid such every minute a viral genome.
- The technique was developed past times Alwine in addition to his colleagues inward 1979
- Technique for detecting specific RNAs separated past times electrophoresis past times hybridization to a labeled deoxyribonucleic acid probe.
Southern blotting | Northern blotting |
Southern cite of inventor | Northern a misnomer |
Separation of deoxyribonucleic acid | Separation of RNA |
Denaturation needed | Denaturation non needed |
Nitrocellulose filter membrane | Amino benzyloxymethyl filter newspaper Membrane |
DNA-DNA Hybridization | RNA-DNA Hybridization |
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