Mr 10 Deviation Betwixt Rapd As Well As Rflp

RAPD ( Random Amplified Polymorphic DNA)

Definition: If primers alongside arbitrary sequences are used for amplification, deoxyribonucleic acid segments to travel amplified volition travel selected at random as well as this provides genuinely random sample of deoxyribonucleic acid markers as well as then is described equally random amplified polymorphic deoxyribonucleic acid (RAPD).

RFLP : Restriction Fragment Length Polymorphism
Definition: It refers to the variation inwards restriction fragment lengths betwixt individuals of a species when cutting yesteryear a restriction enzyme.
If primers alongside arbitrary sequences are used for amplification MR X Difference betwixt RAPD as well as RFLP

RAPD ( Random Amplified Polymorphic DNA)

1. Quantity of deoxyribonucleic acid required for analysis is modest (10-50 ng)

2. Same primers alongside arbitrary sequence tin rank the axe travel used for dissimilar species

3. Fewer steps inwards physical care for thus it is rapid (Five times quicker than RFLP)

4. Technique comparatively less reliable

5. Cannot honour allelic variants

6. 1-10 loci detected

7. Method involves:
       a) extraction of DNA,
       b) amplification yesteryear PCR using random primers,
       c) gel electrophoresis of amplified deoxyribonucleic acid as well as visualisation of markers

RFLP (Restriction Fragment Length Polymorphism)
If primers alongside arbitrary sequences are used for amplification MR X Difference betwixt RAPD as well as RFLP

1. Large quantity of purified deoxyribonucleic acid required i.e. 2-10 ug

2. Different species specific probes are required.

3. Comparatively slower processing due to to a greater extent than steps involved.

4. Technique comparatively to a greater extent than reliable

5. Can honour allelic variants

6. 1-3 loci detected

7. Method involves:
       a) digestion of extracted deoxyribonucleic acid yesteryear restriction enzymes,
       b) gel electrophoresis of fragments,
       c) southern blot yesteryear specific probes as well as detection of specific sequences

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